Home Itens selecionados Provedores de dados OAI Créditos Sobre Ajuda

			Busca avançada
	Provedor de dados Biocell (2) Autor Mroginski,Luis Amado (2) Rey,Hebe Yolanda (2) Flachsland,Eduardo Alberto (1) Fontana,María Laura (1) Surenciski,Mauro Rodrigo (1) Terada,Graciela (1) Mais... Palavra-chave Activated charcoal (1) Calcium alginate (1) Cryoconservation (1) In vitro cultivation (1) Leaflet explants (1) Liquid nitrogen (1) Micropropagation (1) Sucrose (1) Thidiazuron (1) Wild groundnut (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2012 (1) 2009 (1) País Argentina (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Cryopreservation of Cyrtopodium hatschbachii Pabst (Orchidaceae) immature seeds by encapsulation-dehydration Biocell Surenciski,Mauro Rodrigo; Flachsland,Eduardo Alberto; Terada,Graciela; Mroginski,Luis Amado; Rey,Hebe Yolanda. The aim of the present study was to investigate the efficiency of the encapsulation-dehydration technique for cryopreservation of Cyrtopodium hastchbachii Pabst seeds. Immature seeds of this species were cryopreserved by an encapsulation-dehydration technique. Seeds of five immature pods, 120 days after pollination, were encapsulated in 3% calcium alginate matrix and pretreated in liquid medium supplemented with 0.08 M sucrose (24 h), 0.15 M sucrose (24 h), 0.25 M sucrose (48 h), 0.5 M sucrose (24 h) and 0.75 M sucrose (24 h) in shaker at 60 rpm. Alginate beads were dehydrated 5 h in silicagel and immersed in liquid nitrogen for 12 h. Cryopreserved beads were thawed at 30°C for 1 min, rehydrated using the same liquid mediums (0.75 M sucrose (24 h), 0.5 M... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cryoconservation; Liquid nitrogen; Calcium alginate; Activated charcoal; Sucrose. Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000100002 Organogenesis and plant regeneration of Arachis villosa Benth. (Leguminosae) through leaf culture Biocell Fontana,María Laura; Mroginski,Luis Amado; Rey,Hebe Yolanda. With the aim of developing an efficient plant regeneration protocol, leaflet explants of three accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog medium supplemented with different combinations of plant growth regulators: α-naphthalenacetic acid, indole-3-butyric acid, 6-benzylaminopurine, kinetin and thidiazuron. The accession L97 was the only one able to differentiate buds through indirect organogenesis. The most suitable combination for bud regeneration was the basic medium added with 13.62 μM thidiazuron and 4.44 μM 6-benzylaminopurine. These results show the important role of the genotype in morphogenetic responses and the organogenetic effect of thidiazuron in Arachis... Tipo: Info:eu-repo/semantics/article Palavras-chave: In vitro cultivation; Leaflet explants; Micropropagation; Thidiazuron; Wild groundnut. Ano: 2009 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000300006 Registros recuperados: 2 Primeira ... 1 ... Última

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco